A cipher prevents a third party from reading a message. Homophonic substitution enhances its security by providing several cipher symbols for a plaintext letter. On the other hand, polyphonic substitution uses the same symbol for several plaintext letters. This must be confounding even for the intended recipient of the message because there are more than one reading for a given cipher symbol. Practical aspects of polyphonic substitution are reviewed in A. Ross Eckler (1975), "A Readable Polyphonic Cipher" (pdf), which proposes a workable assignment of the letters of the alphabet to figures 1-9 (with "0" reserved for a word break to aid deciphering).
Historically, polyphonic substitution was actually used in 16th-century Italy (see another article) but was not widely used otherwise.
Polyphonic substitution was reportedly used in 1832 by the Duchess of Berry, the widow of the second son of Charles X of France, who had been dethroned by the July Revolution in 1830. In 1841, Edgar Allan Poe challenged the reader of his magazine for sending in a note enciphered in the same manner as the Duchess of Berry's cipher but ended up in complaining that one cryptogram he got had as many as four letters (e, i, s, w) represented by the cipher symbol "i" and a word "wise" might have been enciphered as "iiii" (another article (in Japanese))! (Poe, E.A., "A Few Words on Secret Writing", Graham's Magazine, July 1841, 19:33-38 (online)) David Kahn suggests a possibility that the actual cipher used some diacritics to distinguish letters represented by the same symbol but Poe's source omitted them (Kahn p.787).
Polyphonicity may occur inadvertently. The checkerboard cipher used between George Calvert, English Secretary of State, and Thomas Roe, ambassador to Constantinople in 1623 has polyphonic assignments due to an inappropriate choice of a keyword. The plaintext letters A and C are represented by the same symbol "nu" and similarly for the pairs F/H, L/N, Q/S, and W/Y (see another article).
The present author found that one of the ciphers used by the Duke of Mayenne (then head of the Catholic League), commander de Diou (ambassador in Rome for the Catholic League), and others in 1592-1593 employed polyphonic substitution.
The letters enciphered with this are as follows:
(f.97) from commander de Diou, to "monseigneur le president Janyn [Janin], conseiller d'Estat", Rome, 27 October 1592
(f.101) from Anne de Perusse d'Escars de Givry, Bishop of Lizieux (Wikipedia), to "monseigneur", Rome, 27 October 1592
(f.124) from commander de Diou to Duke of Mayenne, Rome, 12 November 1592
(f.106) from Charles de Lorraine, Duke of Mayenne, to commander de Diou, Soissons, 28 February 1593
(f.108v) from Charles de Lorraine, Duke of Mayenne, to commander de Diou, Soissons, 4 March 1593
(f.211) from Charles de Lorraine, Duke of Mayenne, to commander de Dyou [Diou], Camp of Han, 1 April 1593
(f.176) Baudouin-Desportes to Pope Clement VIII, Paris, 22 July 1593
Deciphered on a separate sheet.
(f.188, 184) Baudouin-Desportes to Bishop of Lizieux, Paris, 22 July 1593
F. 184 is a decipherment of f.188.
(f.186) Baudouin-Desportes to Pietro Aldobrandini, Paris, 22 July 1593
Enciphered passages are undeciphered.
(f.189) Baudouin-Desportes to Hieronimo Frachetta, Paris, 22 July 1593
(f.274) Bishop of Lisieux to Desportes, Sr. de Beuvillier, Rome, July 1593
Neatly written interlined decipherment.
The reconstructed cipher is as follows.
Sharing the same symbol by high frequency letters "e" and "r" means a succession of the symbol three times may easily occur (as in "ere" "rer" "ree" "err"). In addition, due to the similarity of the symbols for "a/n", "c/p", and "d/q" as well as the variation in handwriting may cause confusion when the "4"-like symbol occurs one after another.
The following is a specimen of the use of this cipher (taken from f.108 of BnF fr.3983).
The Duke of Mayenne, who used the above polyphonic cipher in April 1593 in writing to Commander de Diou, used a more conventional homophonic substitution cipher in May 1593 in writing from Paris to the same recipient (BnF fr.3984, ff.7-10).
The following is my reconstruction of this cipher. A symbol like "x" is used to represent "c", "p", and "y". (Right after uploading this reconstruction, I found the original cipher in BnF fr.3995, no.55 (see another article) while browsing the file for a different purpose. At first, I thought the Duke realized the inconvenience of the polyphonic cipher but if the date "1592" on the original cipher is correct, this cipher was made before use of the above polyphonic cipher.)
(The first clue in reconstructing the above cipher came from an undeciphered symbol like "7 with three dots" on the third line. It allowed identifying the symbols for "que" and "je" occurring nearby, which in turn revealed that the symbol "F" near the beginning represented "depeche" rather than its initial letter "d". When "l-a" could be identified near "que", I was sure I was on the right track.)
The decipherment on a separate sheet (f.8) omits the few lines of the second portion in cipher (f.7), which can now be deciphered for the most part as follows.